Abstract: A qualified laboratory lesson is of great importance in science education. In a qualified laboratory lesson, there are so many essential elements such as material, tool and place. One of the most important elements of a qualified laboratory lesson is the equipment used, and usage information and ability of this equipment. Microscope which is frequently used in science education is one of such tools. Microscope usage information is very important for a science educator. Therefore, a teacher who acquired this information and usage ability will be effective in encouraging students to use the microscope correctly, to have interest in science and even to do research in this field. In parallel with this importance, it is thought that the science educators who study in faculties need to have a good ability of microscope usage information. Based on this expectation, the aim of this study is to identify the ability of microscope usage information of the candidate students who study at science teaching department in 2nd grade. This study has been conducted with 80 2nd grade students who study in science teaching department of a university in Black Sea region of Turkey. Microscope usage information scale which was improved by Benzer and Demir in 2014 has been used as obtainment tool in the study. Microscope usage information scale is composed of tripple likert type 20 articles and two open-ended questions. The data which were obtained from likert type articles have been evaluated with frequency calculation whereas the data which were obtained from open-ended questions have been evaluated with content analysis. According to Büyüköztürk et al. (2008), content analysis is defined as a systematic technique where certain words of a text are summarized with smaller content categories through coding. Based on the results that were obtained in the study, interpretations have been made and suggestions have been offered in order to enlighten other researchers.