Abstract: This research examined university students’ understanding about various concepts of population genetics and how they integrate that knowledge to explain the theorie of Evolution. A mixed study method was used to explore the students’ understanding of different factors that affect evolution. Data were collected by questionnaire and interview and were analyzed qualitatively. The results indicate that the majority of students know how can natural selection and mutation affect allele frequency in a population but they have difficulties to understand how act Genetic drift on the evolution of allele frequencies. They had also misconceptions of some concepts related to evolution like speciation. The majority of students don’t believe to Human evolution but they accept the theory of evolution for the other species.